原子力显微镜力谱技术及其在微观生物力学领域的应用

葛林

doi:10.6052/1000-0992-17-011

原子力显微镜力谱技术及其在微观生物力学领域的应用

doi: 10.6052/1000-0992-17-011

葛林^ɛ, ,

NT-MDT中国分公司, 北京 100031

详细信息

通讯作者:
葛林

中图分类号: TH742, Q937;
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出版历程
- 收稿日期: 2017-05-18
- 刊出日期: 2018-02-08

AFM force spectroscopy and its applications on micro biomechanics

GE Lin^{ɛ
, ,}

NT-MDT, China office, Beijing 100031, China

More Information

Corresponding author: GE Lin

摘要

摘要: 基于原子力显微镜技术的力谱技术是一种高灵敏度的力学检测方法.它能够以前所未有的精度, 在微观生物力学领域表征组织、细胞、生物膜、蛋白质、核酸、功能材料等目标对象, 探索其包括形貌、化学信息、导电性、静电力以及生物学特性在内的等信息, 并且能够对其进行分子级别精度的三维操纵. 从而对分子结构与构象变化, 分子间的相互作用以及反应历程实现单分子水平的实时--原位观测, 提供了其他测试方法不能完成的实验设计之可能性.本文首先介绍了原子力显微镜及其力谱技术的原理, 以及影响测量结果的各个参数的物理意义; 其次按照单个目标对象与配对目标对象的区分方式, 详细介绍了力谱技术在微观生物力学各个尺度上的研究进展; 之后介绍了力谱技术结合成像模式下的发展和应用; 最后对设备的改进和本研究领域发展方向进行了展望.
- 原子力显微镜 /
- 生物力学 /
- 分子力谱 /
- 分子间相互作用
Abstract: Atomic force microscopy (AFM) based force spectroscopy is a high-sensitive mechanical detection method. With unprecedented accuracy, this method enables the characterization of a wide range of biological and synthetic bio-interfaces, ranging from tissues, cells, membranes, proteins, nucleic acids to functional materials. Besides the possibility of high resolution imaging of biological samples from the cellular level to the molecular level, AFM-based force spectroscopy allows their mechanical, chemical, conductive or electrostatic, and biological properties to be probed, and helps addressing fundamental challenges that cannot be addressed with other techniques. In this review, we summarize basic and advanced force spectroscopy approaches in micro biomechanics and evaluate their unique advantages and limitations.
- atomic force microscopy /
- biomechanics /
- molecular force spectroscopy /
- intermolecular interactions

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参考文献(355)

[1]	白春礼, 田芳, 罗克. 2000. 扫描力显微术. 北京: 科学出版社, 146-148.
[2]	沈家骢. 2004. 超分子层状结构------组装与功能. 北京: 科学出版社, 78-119.
[3]	Adams J D, Erickson B W, Grossenbacher J, Brugger J, Nievergelt A, Fantner G E.2016. Harnessing the damping properties of materials for high-speed atomic force microscopy. Nature Nanotechnology, 11: 147-151.
[4]	Aggarwal V, Kulothungan S R, Balamurali M M, Saranya S R, Varadarajan R, Ainavarapu S R.2011. Ligand-modulated parallel mechanical unfolding pathways of maltose-binding proteins. Journal of Biological Chemistry, 286: 28056-28065.
[5]	Alegre-Cebollada J, Kosuri P, Rivas-Pardo J A, Fern'{a}ndez J M.2011. Direct observation of disulfide isomerization in a single protein. Nature Chemistry, 3: 882-887.
[6]	Allen S, Chen X, Davies J, Davies M C, Dawkes A C, Edwards J C, Roberts C J, Sefton J, Tendler S J, Williams P M.1997. Detection of antigen-antibody binding events with the atomic force microscope. Biochemistry, 36: 7457-746.
[7]	Alsteens D, Dupres V, Klotz SA, Gaur NK, Lipke P N, Dufrêne Y F.2009. Unfolding individual als5p adhesion proteins on live cells. ACS Nano, 3: 1677-1682.
[8]	Alsteens D, Garcia M C, Lipke P N, Dufrêne Y F.2010. Force-induced formation and propagation of adhesion nanodomains in living fungal cells. Proceedings of the National Academy of Sciences of the United States of America, 107: 20744-20749.
[9]	Alsteens D, Gaub H E, Newton R, Pfreundschuh M, Gerber C, Müller D J.2017a. Atomic force microscopy-based characterization and design of biointerfaces. Nature Reviews Materials, 2: 17008.
[10]	Alsteens D, Newton R, Schubert R, Martinez-Martin D, Delguste M, Roska B, Müller D J.2017b. Nanomechanical mapping of first binding steps of a virus to animal cells. Nature Nanotechnology, 12: 177-183.
[11]	Alsteens D, Pfreundschuh M, Zhang C, Spoerri P M, Coughlin S R, Kobilka B K, Müller D J.2015. Imaging G protein-coupled receptors while quantifying their ligand-binding free-energy landscape. Nature Methods, 12: 845-851.
[12]	Alsteens D, Trabelsi H, Soumillion P, Dufrêne Y F.2013a. Multiparametric atomic force microscopy imaging of single bacteriophages extruding from living bacteria. Nature Communications, 4: 2926.
[13]	Alsteens D, Van Dijck P, Lipke P N, Dufrêne Y F.2013b. Quantifying the forces driving cell-cell adhesion in a fungal pathogen. Langmuir, 29: 13473-13480.
[14]	Amo C A, Garcia R.2016. Fundamental high-speed limits in single-molecule single-cell and nanoscale force spectroscopies. ACS Nano, 10: 7117-7124.
[15]	Ando T, Uchihashi T, Kodera N.2013. High-speed AFM and applications to biomolecular systems. Annual Review of Biophysics, 42: 393-414.
[16]	Ardell D H, Andersen S O.2001. Tentative identification of a resilin gene in Drosophila melanogaster. Insect Biochemistry and Molecular Biology, 31: 965970.
[17]	Azeloglu E U, Costa K D.2009. Dynamic AFM elastography reveals phase dependent mechanical heterogeneity of beating cardiac myocytes. Proceedings of Engineering in Medicine and Biology Society, 2009: 7180-7183.
[18]	Balamurali M M, Sharma D, Chang A, Khor D, Chu R, Li H.2008. Recombination of protein fragments: A promising approach toward engineering proteins with novel nanomechanical properties. Protein Science, 17: 1815-1826.
[19]	B'{a}lint Z, Krizbai IA, Wilhelm I, Farkas AE, P'{a}rducz A, Szegletes Z, V'{a}r'{o} G.2007. Changes induced by hyperosmotic mannitol in cerebral endothelial cells: An atomic force microscopic study. European Biophysics Journal, 36: 113-120.
[20]	Baltrukovich B.2008. Mechanical unfolding of membrane proteins captured with single-molecule AFM techniques. [PhD Thesis]. Germany: Technische Universität Dresden.
[21]	Barattin R, Voyer N.2008. Chemical modifications of AFM tips for the study of molecular recognition events. Chemical Communications, 13: 1513-1532.
[22]	Baumann F, Bauer M S, Milles L F, Alexandrovich A, Gaub H E, Pippig D A.2016. Monovalent Strep-Tactin for strong and site-specific tethering in nanospectroscopy. Nature Nanotechnology, 11: 89-94.
[23]	Baumgartner W, Hinterdorfer P, Ness W, Raab A, Vestweber D, Schindler H, Drenckhahn D.2000. Cadherin interaction probed by atomic force microscopy. Proceedings of the National Academy of Sciences of the United States of America, 97: 4005-4010.
[24]	Beaussart A, Abell'{a}n-Flos M, El-Kirat-Chatel S, Vincent S P, Dufrêne Y F.2016. Force nanoscopy as a versatile platform for quantifying the activity of antiadhesion compounds targeting bacterial pathogens. Nano Letters, 16: 1299-1307.
[25]	Beaussart A, El{-}Kirat-Chatel S, Fontaine T, Latge J P, Dufrêne Y F.2015. Nanoscale biophysical properties of the cell surface galactosaminogalactan from the fungal pathogen aspergillus fumigatus. Nanoscale, 7: 14996-15004.
[26]	Beaussart A, El-Kirat-Chatel S, Herman P, Alsteens D, Mahillon J, Hols P, Dufrêne Y F.2013. Single-cell force spectroscopy of probiotic bacteria. Biophysical Journal, 104: 1886-1892.
[27]	Beauzamy L, Derr J, Boudaoud A.2015. Quantifying hydrostatic pressure in plant cells by using indentation with an Atomic Force Microscope. Biophysical Journal, 108: 2448-2456.
[28]	Becker N, Oroudjev E, Mutz S, Cleveland J P, Hansma P K, Hayashi C Y, Makarov D E, Hansma H G.2003. Molecular nanosprings in spider capture-silk threads. Nature Materials, 2: 278-283.
[29]	Bell G I.1978. Models for the specific adhesion of cells to cells. Science, 200: 618-627.
[30]	Benoit M, Gabriel D, Gerisch G, Gaub H E.2000. Discrete interactions in cell adhesion measured by single-molecule force spectroscopy. Nature Cell Biology, 2: 313-317.
[31]	Berkovich R, Hermans R I, Popa I, Stirnemann G, Garcia-Manyes S, Berne B J, Fern'{a}ndez J M.2012. Rate limit of protein elastic response is tether dependent. Proceedings of the National Academy of Sciences of the United States of America, 109: 14416-14421.
[32]	Bertz M, Kunfermann A, Rief M.2008. Navigating the folding energy landscape of green fluorescent protein. Angewandte Chemie International Edition, 47: 8192-8195.
[33]	Bertz M, Wilmanns M, Rief M.2009. The titin-telethonin complex is a directed superstable molecular bond in the muscle Z-disk. Proceedings of the National Academy of Sciences of the United States of America, 106: 13307-13310.
[34]	Binnig G, Quate C F, Gerber C.1986. Atomic force microscope. Physical Review Letters, 56: 930-933.
[35]	Bippes C A.2010. Investigation of biological macromolecules using atomic force microscope-based techniques. [PhD Thesis]. Germany: Technische Universität Dresden.
[36]	Bippes C A, Ge L, Meury M, Harder D, Ucurum Z, Daniel H, Fotiadis D, Müller D J.2013. Peptide transporter DtpA has two alternate conformations one of which is promoted by inhibitor binding. Proceedings of the National Academy of Sciences of the United States of America, 110: 3978-3986.
[37]	Bippes C A, Müller D J.2011. High-resolution atomic force microscopy and spectroscopy of native membrane proteins. Reports on Progress in Physics, 74: 86601.
[38]	Bornschlögl T, Anstrom D M, Mey E, Dzubiella J, Rief M, Forest K T.2009a. Tightening the knot in phytochrome by single-molecule atomic force microscopy. Biophysical Journal, 96: 1508-1514.
[39]	Bornschlögl T, Woehlke G, Rief M.2009b. Single molecule mechanics of the kinesin neck. Proceedings of the National Academy of Sciences of the United States of America, 106: 6992-6997.
[40]	Brockwell D J, Paci E, Zinober R C, Beddard G S, Olmsted P D, Smith D A, Perham R N, Radford S E.2003. Pulling geometry defines the mechanical resistance of a beta-sheet protein. Nature Structural & Molecular Biology, 10: 731-737.
[41]	Brujić J, Hermans R, Walther K, Fern'{a}ndez J M.2006. Single-molecule force spectroscopy reveals signatures of glassy dynamics in the energy landscape of ubiquitin. Nature Physics, 2: 282-286.
[42]	Bu T, Wang H C, Li H.2012. Single molecule force spectroscopy reveals critical roles of hydrophobic core packing in determining the mechanical stability of protein GB1. Langmuir, 28: 12319-12325.
[43]	Bull M S, Sullan R M, Li H, Perkins T T.2014. Improved single molecule force spectroscopy using micromachined cantilevers. ACS Nano, 8: 4984-4995.
[44]	Buriak J M.2002. Organometallic chemistry on silicon and germanium surfaces. Chemical Reviews, 102: 1271-1308.
[45]	Bustamante C, Marko J F, Siggia E D, Smith S.1994. Entropic elasticity of lambda-phage DNA. Science, 265: 1599-1600.
[46]	Butt H J, Cappella B, Kappl M.2005. Force measurements with the atomic force microscope: technique interpretation and applications. Surface Science Reports, 59: 1-152.
[47]	Calzado-Martín A, Encinar M, Tamayo J, Calleja M, Paulo A S.2016. Effect of actin organization on the stiffness of living breast cancer cells revealed by peak-force modulation atomic force microscopy. ACS Nano, 10: 3365-3374.
[48]	Cao Y, Balamurali M, Sharma D, Li H.2007. A functional single-molecule binding assay via force spectroscopy. Proceedings of the National Academy of Sciences of the United States of America, 104: 15677-15681.
[49]	Cao Y, Li H.2011a. Dynamics of protein folding and cofactor binding monitored by single-molecule force spectroscopy. Biophysical Journal, 101: 2009-2017.
[50]	Cao Y, Li H.2011b. Single-molecule force-clamp spectroscopy dwell time analysis and practical considerations. Langmuir, 27: 1440-1447.
[51]	Cao Y, Li H.2007. Polyprotein of GB1 is an ideal artificial elastomeric protein. Nature Methods, 6: 109-114.
[52]	Cao Y, Li H.2008. Engineered elastomeric proteins with dual elasticity can be controlled by a molecular regulator. Nature Nanotechnology, 3: 512-516.
[53]	Cao Y, Yoo T, Li H.2008. Single molecule force spectroscopy reveals engineered metal chelation is a general approach to enhance mechanical stability of proteins. Proceedings of the National Academy of Sciences of the United States of America, 105: 11152-11157.
[54]	Cappella B, Dietler G.1999. Force-distance curves by atomic force microscopy. Surface Science Reports, 34: 1-104.
[55]	Carl P, Schillers H.2008. Elasticity measurement of living cells with an atomic force microscope: Data acquisition and processing. European Journal of Applied Physiology, 457: 551-559.
[56]	Carrion-Vazquez M, Li H, Lu H, Marszalek P E, Oberhauser A F, Fern'{a}ndez J M.2003. The mechanical stability of ubiquitin is linkage dependent. Nature Structural & Molecular Biology, 10: 738-743.
[57]	Carvalho F A, Connell S, Miltenberger-Miltenyi G, Pereira S V, Tavares A, AriËns R A, Santos N C.2010. Atomic force microscopy-based molecular recognition of a fibrinogen receptor on human erythrocytes. ACS Nano, 4: 4609-4620.
[58]	Carvalho F A, Santos N C.2012. Atomic force microscopy-based force spectroscopy-biological and biomedical applications. IUBMB Life, 64: 465-472.
[59]	Cassina V, Torsello A, Tempestini A, Salerno D, Brogioli D, Tamiazzo L, Bresciani E, Martinez J, Fehrentz JA, Verdi'{e} P, Omeljaniuk R J, Possenti R, Rizzi L, Locatelli V, Mantegazza F.2013. Biophysical characterization of a binding site for tlqp-21 a naturally occurring peptide which induces resistance to obesity. Biomembranes, 1828: 455-460.
[60]	Cattin C J, Düggelin M, Martinez-Martin D, Gerber C, Müller D J, Stewart M P.2015. Mechanical control of mitotic progression in single animal cells. Proceedings of the National Academy of Sciences of the United States of America, 112: 11258-11263.
[61]	Chaudhuri O, Parekh S H, Lam W A, Fletcher D A.2009. Combined atomic force microscopy and side-view optical imaging for mechanical studies of cells. Nature Methods, 6: 383-387.
[62]	Chiaruttini N, Redondo-Morata L, Colom A, Humbert F, Lenz M, Scheuring S, Roux A.2015. Relaxation of loaded ESCRT-III spiral springs drives membrane deformation. Cell, 163: 866-879.
[63]	Chiti F, Dobson C M.2006. Protein misfolding functional amyloid and human disease. Annual Review of Biochemistry, 75: 333-366.
[64]	Chouinard J A, Grenier G, Khalil A, Vermette P.2008. Oxidized-LDL induce morphological changes and increase stiffness of endothelial cells. Experimental Cell Research , 314: 3007-3016.
[65]	Chtcheglova L A, Waschke J, Wildling L, Drenckhahn D, Hinterdorfer P.2007. Nano-scale dynamic recognition imaging on vascularendothelial cells. Biophysical Journal, 93: 43052.
[66]	Cisneros D A, Hung C, Franz C M, Müller D J.2006. Observing growth steps of collagen self-assembly by time-lapse high-resolution atomic force microscopy. Journal of Structural Biology, 154: 232-245.
[67]	Cordes T, Strackharn M, Stahl S W, Summerer W, Steinhauer C, Forthmann C, Puchner E M, Vogelsang J, Gaub H E, Tinnefeld P.2010. Resolving single-molecule assembled patterns with superresolution blink-microscopy. Nano Letters, 10: 645-651.
[68]	Costa K D.2003. Single-cell elastography: Probing for disease with the atomic force microscope. Disease Markers, 2: 139-154.
[69]	Cross S E, Jin Y S, Rao J, Gimzewski J K.2007. Nanomechanical analysis of cells from cancer patients. Nature Nanotechnology, 2: 780-783.
[70]	Cui S, Albrecht C, Kühner F, Gaub H E.2006. Weakly bound water molecules shorten single-stranded DNA. Journal of the American Chemical Society, 128: 6636-6639.
[71]	Cui S, Yu J, Kühner F, Schulten K, Gaub H E.2007. Double-stranded DNA dissociates into single strands when dragged into a poor solvent. Journal of the American Chemical Society, 129: 14710-14716.
[72]	Czajkowsky D M, Hotze E M, Shao Z, Tweten R K.2004. Vertical collapse of a cytolysin prepore moves its transmembrane beta-hairpins to the membrane. EMBO Journal, 23: 3206-3215.
[73]	Dague E, Alsteens D, Latg'{e} J P, Verbelen C, Raze D, Baulard A R, Dufrêne Y F.2007. Chemical force microscopy of single live cells. Nano Letters, 7: 3026-3030.
[74]	Damaghi M, Köster S, Bippes C A, Yildiz O, Müller D J.2011. One β hairpin follows the other exploring refolding pathways and kinetics of the transmembrane b-barrel protein OmpG. Angewandte Chemie International Edition, 50: 7422-7424.
[75]	Dammer U.1995. Binding strength between cell adhesion proto glycols measured by atomic force microscope. Science, 267: 1173-1176.
[76]	Derjaguin B V, Müller V M, Toporov Y.1975. Effect of contact deformations on the adhesion of particles. Journal of Colloid and Interface Science, 53: 314-326.
[77]	Dietz H, Berkemeier F, Bertz M, Rief M .2006. Anisotropic deformation response of single protein molecules. Proceedings of the National Academy of Sciences of the United States of America, 103: 12724-12728.
[78]	Dobson C M.2003. Protein folding and misfolding. Nature, 426: 884-890.
[79]	Dokukin M E, Sokolov I.2012. On the measurements of rigidity modulus of soft materials in nanoindentation experiments at small depth. Macromolecules, 45: 4277-4288.
[80]	Dong M, Husale S, Sahin O.2009. Determination of protein structural flexibility by microsecond force spectroscopy. Nature Nanotechnology, 4: 514-517.
[81]	Dong M, Sahin O.2011. A nanomechanical interface to rapid single-molecule interactions. Nature Communications, 2: 247.
[82]	Ducker W A, Senden T J, Pashley R M.1991. Direct measurement of colloidal forces using an atomic force microscope. Nature, 353: 239-241.
[83]	Dudko O K, Hummer G, Szabo A.2008. Theory analysis and interpretation of single-molecule force spectroscopy experiments. Proceedings of the National Academy of Sciences of the United States of America, 105: 15755-15760.
[84]	Dufrêne Y F, Martínez-Martín D, Medalsy I, Alsteens D, Müller D J.2013. Multiparametric imaging of biological systems by force-distance curve-based AFM. Nature Methods, 10: 847-854.
[85]	Dupres V, Alsteens D, Wilk S, Hansen B, Heinisch J J, Dufrêne Y F.2009. The yeast Wsc1 cell surface sensor behaves like a nanospring in vivo. Nature Chemical Biology, 5: 857-862.
[86]	Dupres V, Menozzi F D, Locht C, Clare B H, Abbott N L, Cuenot S, Bompard C, Raze D, Dufrêne Y F.2005. Nanoscale mapping and functional analysis of individual adhesins on living bacteria. Nature Methods, 2: 515-520.
[87]	Dupres V, Verbelen C, Dufrêne Y F.2007. Probing molecular recognition sites on biosurfaces using AFM. Biomaterials, 28: 2393-2402.
[88]	Ebner A, Wildling L, Kamruzzahan A S, Rankl C, Wruss J, Hahn C D, Hölzl M, Zhu R, Kienberger F, Blaas D, Hinterdorfer P, Gruber H J.2007. A new simple method for linking of antibodies to atomic force microscopy tips. Bioconjugate Chemistry, 18: 1176-1184.
[89]	El-Kirat-Chatel S, Beaussart A, Alsteens D, Sarazin A, Jouault T, Dufrêne Y F.2013. Single-molecule analysis of the major glycopolymers of pathogenic and non-pathogenic yeast cells. Nanoscale, 5: 4855-4863.
[90]	Elvin C M, Carr A G, Huson M G, Maxwell J M, Pearson R D, Vuocolo T, Liyou N E, Wong D C, Merritt D J, Dixon N E .2005. Synthesis and properties of crosslinked recombinant pro-resilin. Nature, 437: 999-1002.
[91]	Erdmann M, David R, Fornof A, Gaub H E.2010. Electrically induced bonding of DNA to gold. Nature Chemistry, 2: 745-749.
[92]	Evans E A, Calderwood D A.2007. Forces and bond dynamics in cell adhesion. Science, 316: 1148-1153.
[93]	Evans E, Ritchie K.1997. Dynamic strength of molecular adhesion bonds. Biophysical Journal, 72: 1541-1555.
[94]	Fischer-Friedrich E, Hyman A A, Julicher F, Müller D J, Helenius J.2014. Quantification of surface tension and internal pressure generated by single mitotic cells. Scientific Reports, 4: 6213.
[95]	Fischer-Friedrich E, Toyoda Y, Cattin C J, Müller D J, Hyman A A, Jülicher F.2016. Rheology of the active cell cortex in mitosis. Biophysical Journal, 111: 589-600.
[96]	Fisher T E, Oberhauser A F, Carrion-Vazquez M, Marszalek P E, Fern'{a}ndez J M.1999. The study of protein mechanics with the atomic force microscope. Trends in Biochemical Sciences, 24: 379-384.
[97]	Florin E L, Moy V T, Gaub H E.1994. Adhesion forces between individual ligand-receptor pairs. Science, 264: 415-417.
[98]	Formosa-Dague C, Speziale P, Foster T J, Geoghegan J A, Dufrêne Y F.2016. Zinc-dependent mechanical properties of Staphylococcus aureus biofilm-forming surface protein SasG. Proceedings of the National Academy of Sciences of the United States of America, 113: 410-415.
[99]	Francius G, Lebeer S, Alsteens D, Wildling L, Gruber H J, Hols P, De Keersmaecker S, Vanderleyden J, Dufrêne Y F.2008. Detection localization and conformational analysis of single polysaccharide molecules on live bacteria. ACS Nano, 2: 1921-1929.
[100]	Franz C M, Müller D J.2005. Analysing focal adhesion structure by AFM. Journal of Cell Science, 118: 5315-5323.
[101]	Friddle R W, Noy A, De Yoreo J J.2012. Interpreting the widespread nonlinear force spectra of intermolecular bonds. Proceedings of the National Academy of Sciences of the United States of America, 109: 13573-13578.
[102]	Friedrichs J, Helenius J, Müller D J.2010a. Quantifying cellular adhesion to extracellular matrix components by single-cell force spectroscopy. Nature Protocols, 5: 1353-1361.
[103]	Friedrichs J, Helenius J, Müller D J.2010b. Stimulated single-cell force spectroscopy to quantify cell adhesion receptor crosstalk. Proteomics, 10: 1455-1462.
[104]	Friedrichs J, Werner C, Müller D J.2013. Quantifying cellular adhesion to covalently immobilized extracellular matrix proteins by single-cell force spectroscopy. Methods in Molecular Biology, 1046: 19-37.
[105]	Friedrichs J, Taubenberger A, Franz C M, Müller D J.2007. Cellular remodelling of individual collagen fibrils visualized by time-lapse AFM. Journal of Molecular Biology, 372: 594-607.
[106]	Frisbie C D, Rozsnyai L F, Noy A, Wrighton M S, Lieber C M.1994. Functional group imaging by chemical force microscopy. Science, 265: 2071-2074.
[107]	Garcia R, Knoll A W, Riedo E.2014. Advanced scanning probe lithography. Nature Nanotechnology, 9: 577-587.
[108]	Garcia-Manyes S, Liang J, Szoszkiewicz R, Kuo T L, Fern'{a}ndez J M.2009. Force-activated reactivity switch in a bimolecular chemical reaction. Nature Chemistry, 1: 236-242.
[109]	Garcia-Manyes S, Giganti D, Badilla C L, Lezamiz A, Perales-Calvo J, Beedle A E, Fern'{a}ndez J M.2015. Single molecule force spectroscopy predicts a misfolded domain-swapped conformation in human γD-crystallin. Journal of Biological Chemistry, 291: 4226-4235.
[110]	Gaub B M, Kaul C, Zimmermann J L, Carell T, Gaub H E.2009. Switching the mechanics of dsDNA by Cu salicylic aldehyde complexation. Nanotechnology, 20: 434002.
[111]	Gavara N, Chadwick R S.2012. Determination of the elastic moduli of thin samples and adherent cells using conical atomic force microscope tips. Nature Nanotechnology, 7: 733-736.
[112]	Ge L, Jin G, Fang X.2012. Investigation of the Interaction between a Bivalent Aptamer and Thrombin by AFM. Langmuir, 28: 707-713.
[113]	Ge L, Villinger S, Mari S A, Giller K, Griesinger C, Becker S, Müller D J, Zweckstetter M.2016. Molecular plasticity of the human voltage-dependent anion channel embedded into a membrane. Structure, 24: 585-594.
[114]	Gilbert Y, Deghorain M, Wang L, Xu B, Pollheimer P D, Gruber H J, Errington J, Hallet B, Haulot X, Verbelen C, Hols P, Dufrêne Y F.2007. single-molecule force spectroscopy and imaging of the vancomycin/d alad ala interaction. Nano Letters, 7: 796-801.
[115]	Gonnermann C, Huang C, Becker S F, Stamov D R, Wedlich D, Kashef J, Franz C M.2015. Quantitating membrane bleb stiffness using AFM force spectroscopy and an optical sideview setup. Integrative Biology, 7: 356-363.
[116]	Grandbois M, Dettmann W, Benoit M, Gaub H E.2000. Affinity imaging of red blood cells using an atomic force microscope. Journal of Histochemistry and Cytochemistry, 48: 719-724.
[117]	Granzier H L, Pollack G H.2000. Elastic Filaments of the Cell. Springer Science/Busisness Media LLC.
[118]	Gudzenko T, Franz C M.2015. Studying early stages of fibronectin fibrillogenesis in living cells by atomic force microscopy. Molecular Biology of the Cell, 26: 3190-3204.
[119]	Guerette P A, Ginzinger D G, Weber B H, Gosline J M.1996. Silk properties determined by gland-specific expression of a spider fibroin gene family. Science, 272: 112-115.
[120]	Guo S, Zhu X, Ja馽zewski D, Lee S S, He T, Teo S L, Vancso G J.2016. Measuring protein isoelectric points by AFM-based force spectroscopy using trace amounts of sample. Nature Nanotechnology, 11: 817-823.
[121]	Guz N, Dokukin M, Kalaparthi V, Sokolov I.2014. If cell mechanics can be described by elastic modulus: study of different models and probes used in indentation experiments. Biophys Journal, 107: 564-575.
[122]	Hansma P K, Schitter G, Fantner G E, Prater C.2006. High-speed atomic force microscopy. Science, 314: 601-602.
[123]	He C, Hu C, Hu X, Hu X, Xiao A, Perkins T T, Li H.2015. Direct observation of the reversible two-state unfolding and refolding of an ab protein by single-molecule atomic force microscopy. Angewandte Chemie International Edition, 54: 9921-9925.
[124]	He C, Lamour G, Xiao A, Gsponer J, Li H.2014. Mechanically tightening a protein slipknot into a trefoil knot. Journal of the American Chemical Society, 136: 11946-11955.
[125]	Hecht F M, Rheinlaender J, Schierbaum N, Goldmann W H, Fabry B, Schäffer T E.2015. Imaging viscoelastic properties of live cells by AFM: Power-law rheology on the nanoscale. Soft Matter, 11: 4584-4591.
[126]	Heim M, Keerl D, Scheibel T.2009. Spider silk: from soluble protein to extraordinary fiber. Angewandte Chemie International Edition, 48: 3584-3596.
[127]	Helenius J, Heisenberg C P, Gaub H E, Müller D J.2008. Single-cell force spectroscopy. Journal of Cell Science, 121: 1785-1791.
[128]	Hernandez-Ramon E E, Burns J E, Zhang W, Walker H F, Allen S, Antson A A, Maitland N J.2008. Dimerization of the human papillomavirus Type 16 E2 N terminus results in DNA looping within the upstream regulatory region. Journal of Virology, 82: 4853-4861.
[129]	Herruzo E T, Perrino A P, Garcia R.2014. Fast nanomechanical spectroscopy of soft matter. Nature Communications, 5: 3126.
[130]	Heu C, Berquand A, Elie-Caille C, Nicod L.2012. Glyphosate-induced stiffening of HaCaT keratinocytes, a peak force tapping study on living cells. Journal of Structural Biology, 178: 1-7.
[131]	Heucke S F, Baumann F, Acuna G P, Severin P M, Stahl S W, Strackharn M, Stein I H, Altpeter P, Tinnefeld P, Gaub H E.2014. Placing individual molecules in the center of nanoapertures. Nano Letters, 14: 391-395.
[132]	Hinterdorfer P, Baumgartner W, Gruber H J, Schilcher K, Schindler H.1996. Detection and localization of individual antibody-antigen recognition events by atomic force microscopy. Proceedings of the National Academy of Sciences of the United States of America, 93: 3477-3481.
[133]	Hinterdorfer P, Dufrêne Y F.2006. Detection and localization of single molecular recognition events using atomic force microscopy. Nature Methods, 3: 347-355.
[134]	Horton M, Charras G, Lehenkari P.2002. Analysis of ligand-receptor interactions in cells by atomic force microscopy. Journal of Receptor and Signal Transduction Research, 22: 169-190.
[135]	Hsu S H, Lo Y Y, Tung J Y, Ko Y C, Sun Y J, Hung C C, Yang C W, Tseng F G, Fu C C, Pan R L.2010. Leptospiral outer membrane lipoprotein lipl32 binding on toll-like receptor 2 of renal cells as determined with an atomic force microscope. Biochemistry, 49: 5408-5417.
[136]	Hugel T, Holland N B, Cattani A, Moroder L, Seitz M, Gaub H E.2002. Single-molecule optomechanical cycle. Science, 296: 1103-1106.
[137]	Hugel T, Seitz M.2001. The study of molecular interactions by AFM force spectroscopy. Macromolecular Rapid Communications, 22: 989-1016.
[138]	Ido S, Kimiya H, Kobayashi K, Kominami H, Matsushige K, Yamada H.2014. Immunoactive two-dimensional self-assembly of monoclonal antibodies in aqueous solution revealed by atomic force microscopy. Nature Materials, 13: 264-270
[139]	Ido S, Kimura K, Oyabu N, Kobayashi K, Tsukada M, Matsushige K, Yamada H.2013. Beyond the helix pitch: Direct visualization of native DNA in aqueous solution. ACS Nano, 7: 1817-1822.
[140]	Iyer S, Gaikwad R M, Subba-Rao V, Woodworth C D, Sokolov I.2009. Atomic force microscopy detects differences in the surface brush of normal and cancerous cells. Nature Nanotechnology, 4: 389-393.
[141]	Janmey P A, Georges P C, Hvidt S.2007. Basic rheology for biologists. Methods in Cell Biology, 83: 3-27.
[142]	Janovjak H, Knaus H, Müller D J.2007. Transmembrane helices have rough energy surfaces. Journal of the American Chemical Society, 129: 246-247.
[143]	Janovjak H, Struckmeier J, Hubain M, Kedrov A, Kessler M, Müller D J.2004. Probing the energy landscape of the membrane protein bacteriorhodopsin. Structure, 12: 871-879.
[144]	Janshoff A, Neitzert M, Oberdorfer Y, Fuchs H.2000. Force spectroscopy of molecular systems-single molecule spectroscopy of polymers and biomolecules. Angewandte Chemie International Edition, 39: 3212-3237.
[145]	Jiang Y, Qin F, Li Y, Fang X, Bai C.2004. Measuring specific interaction of transcription factor ZmDREB1A with its DNA responsive element at the molecular level. Nucleic Acids Research, 32: e101.
[146]	Jiang Y, Zhu C, Ling L, Wan L, Fang X, Bai C.2003. Specific aptamer-protein interaction studied by atomic force microscopy. Analytical Chemistry, 75: 2112-2116.
[147]	Jin H, Ma S, Song B, Ma L, Pi J, Chen X, Chen Y, Cai J.2011. Liposome impaired the adhesion and spreading of HEK293 cells: An AFM study. Scanning, 33: 413-418.
[148]	Johnson K L.1987. Contact Mechanics. Cambridge: Cambridge University Press.
[149]	Johnson K L, Kendall K, Roberts A D.1971. Surface energy and the contact of elastic solids. Proceedings of the Royal Society, 324: 301-313.
[150]	Junker J, Rief M.2010. Evidence for a Broad Transition-State Ensemble in Calmodulin Folding from Single-Molecule Force Spectroscopy. Angewandte Chemie International Edition, 49: 3306-3309.
[151]	Karner A, Nimmervoll B, Plochberger B, Klotzsch E, Horner A, Knyazev D G, Kuttner R, Winkler K, Winter L, Siligan C, Ollinger N, Pohl P, Preiner J.2016. Tuning membrane protein mobility by confinement into nanodomains. Nature Nanotechnology, 12: 260-266.
[152]	Kasas S, Dietler G.2008. Probing nanomechanical properties from biomolecules to living cells. Pfl"{ugers Archiv: European Journal of Physiology}, 456: 13-27.
[153]	Kawamura S, Colozo A T, Ge L, Müller D J, Park P S.2012. Structural energetic and mechanical perturbations in rhodopsin mutant that causes congenital stationary night blindness. Journal of Biological Chemistry, 287: 21826-21835.
[154]	Kedrov A, Appel M, Baumann H, Ziegler C, Müller D J.2008. Examining the dynamic energy landscape of an antiporter upon inhibitor binding. Journal of Molecular Biology, 375: 1258-1266.
[155]	Kedrov A, Janovjak H, Ziegler C, Kuhlbrandt W, Müller D J.2006. Observing folding pathways and kinetics of a single sodium-proton antiporter f rom Escherichia coli. Journal of Molecular Biology, 355: 2-8.
[156]	Kessler M, Gottschalk K E, Janovjak H, Müller D J, Gaub H E.2006. Bacteriorhodopsin folds into the membrane against an external force. Journal of Molecular Biology, 357: 644-654.
[157]	Kim D, Sahin O.2015. Imaging and three-dimensional reconstruction of chemical groups inside a protein complex using atomic force microscopy. Nature Nanotechnology, 10: 264-269.
[158]	Kim H, Arakawa H, Hatae N, Sugimoto Y, Matsumoto O, Osada T, Ichikawa A, Ikai A.2006. Quantification of the number of EP3 receptors on a living cho cell surface by the AFM. Ultramicroscopy, 106: 652-662.
[159]	Kim H, Arakawa H, Osada T, Ikai A.2003. Quantification of cell adhesion force with AFM: Distribution of vitro nectin receptors on a living MC3T3-E1 cell. Ultramicroscopy, 97: 359-363.
[160]	King G M, Carter A R, Churnside A B, Eberle L S, Perkins T T.2009. Ultrastable atomic force microscopy: Atomic-scale stability and registration in ambient conditions. Nano Letters, 9: 1451-1456.
[161]	Kodera N, Yamamoto D, Ishikawa R, Ando T.2010. Video imaging of walking myosin V by high-speed atomic force microscopy. Nature, 468: 72-76.
[162]	Kopycinska-Müller M, Geiss R H, Hurley D C.2006. Contact mechanics and tip shape in AFM-based nanomechanical measurements. Ultramicroscopy, 106: 466-474.
[163]	Koti Ainavarapu S R, Wiita A P, Dougan L, Uggerud E, Fern'{a}ndez J M.2008. Single-molecule force spectroscopy measurements of bond elongation during a bimolecular reaction. Journal of the American Chemical Society, 130: 6479-6487.
[164]	Krasnoslobodtsev A V, Shlyakhtenko L S, Lyubchenko Y L.2007. Probing interactions within the synaptic DNA-SfiI complex by AFM force spectroscopy. Journal of Molecular Biology, 365: 1407-1416.
[165]	Krautbauer R, Clausen-Schaumann H, Gaub H E.2000. Cisplatin changes the mechanics of single DNA molecules. Angewandte Chemie International Edition, 39: 3912-3915.
[166]	Krautbauer R, Fischerländer S, Allen S, Gaub H E.2002a. Mechanical fingerprints of DNA drug complexes. Single Molecules, 3: 97-103.
[167]	Krautbauer R, Pope L H, Schrader T E, Allen S, Gaub H E.2002b. Discriminating small molecule DNA binding modes by single molecule force spectroscopy. FEBS Letters, 510: 154-158.
[168]	Krautbauer R, Rief M, Gaub H E.2003. Unzipping DNA oligomers. Nano Letters, 3: 493-496.
[169]	Krieg M, Arboleda-Estudillo Y, Puech P H, Käfer J, Graner F, Müller D J, Heisenberg C P.2008a. Tensile forces govern germ-layer organization in zebrafish. Nature Cell Biology, 10: 429-436.
[170]	Krieg M, Dunn A R, Goodman M B.2014. Mechanical control of the sense of touch by beta-spectrin. Nature Cell Biology, 16: 224-233.
[171]	Krieg M, Helenius J, Heisenberg C P, Müller D J.2008b. A bond for a lifetime: employing membrane nanotubes from living cells to determine receptor-ligand kinetics. Angewandte Chemie International Edition, 47: 9775-9777.
[172]	Kühner F, Erdmann M, Sonnenberg L, Serr A, Morfill J, Gaub H E.2006. Friction of single polymers at surfaces. Langmuir, 22: 11180-11186.
[173]	Kufer S K, Strackharn M, Stahl S W, Gumpp H, Puchner E M, Gaub H E.2009. Optically monitoring the mechanical assembly of single molecules. Nature Nanotechnology, 4: 45-49.
[174]	Kuo T L, Garcia-Manyes S, Li J, Barel I, Lu H, Berne B J, Urbakh M, Klafter J, Fern'{a}ndez J M.2010. Probing static disorder in Arrhenius kinetics by single-molecule force spectroscopy. Proceedings of the National Academy of Sciences of the United States of America, 107: 11336-11340.
[175]	Kuznetsov Y G, McPherson A.2011. Atomic force microscopy in imaging of viruses and virus-infected cells. Microbiology and Molecular Biology Reviews, 75: 268-285.
[176]	Kuznetsova T G, Starodubtseva M N, Yegorenkov N I, Chizhik S A, Zhdanov R I.2007. Atomic force microscopy probing of cell elasticity. Micron, 38: 824-833.
[177]	Ladjal H, Hanus J, Pillarisetti A, Keefer C, Ferreira A, Desai J.2009. Atomic force microscopy-based single-cell indentation: experimentation and finite element simulation. Proceedings of IEEE// International Conference on Intelligent Robots and Systems, StLouis, USA: 1326-1332.
[178]	Lam W, Rosenbluth M, Fletcher D.2007. Chemotherapy exposure increases leukemia cell stiffness. Blood, 109: 3505-3508.
[179]	Lamour G, Kirkegaard J B, Li H, Knowles T P, Gsponer J.2014. Easyworm: An open-source software tool to determine the mechanical properties of worm-like chains. Source Code for Biology and Medicine, 9: 16.
[180]	Le Cigne A, Chi`{e}ze L, Beaussart A, El-Kirat-Chatel S, Dufrêne Y F, Dedieu S, Schneider C, Martiny L, Devy J, Molinari M.2016. Analysis of the effect of LRP-1 silencing on the invasive potential of cancer cells by nanomechanical probing and adhesion force measurements using atomic force microscopy. Nanoscale, 8: 7144-7154.
[181]	Lee C K, Wang Y M, Huang L S, Lin S.2007. Atomic force microscopy: Determination of unbinding force, off rate and energy barrier for protein-ligand interaction. Micron, 38: 446-461.
[182]	Lehenkari P P, Horton M A.1999. Single integrin molecule adhesion forces in intact cells measured by AFM. Biochemical and Biophysical Research Communications, 259: 645-650.
[183]	Lehto T, Miaczynska M, Zerial M, Müller D J, Severin F.2003. Observing the growth of individual actin filaments in cell extracts by time-lapse atomic force microscopy. FEBS Letters, 551: 25-28.
[184]	Li H.2007. Engineering proteins with tailored nanomechanical properties a single molecule approach. Organic & Biomolecular Chemistry, 5: 3399-3406.
[185]	Li H, Carrion-Vazquez M, Oberhauser A F, Marszalek P E, Fern'{a}ndez J M.2000. Point mutations alter the mechanical stability of immunoglobulin modules. Nature Structural & Molecular Biology, 7: 1117-1120.
[186]	Li H, Linke W A, Oberhauser A F, Carrion-Vazquez M, Kerkvliet J G, Lu H, Marszalek P E, Fern'{a}ndez J M.2002. Reverse engineering of the giant muscle protein titin. Nature, 418: 998-1002.
[187]	Li H, Rief M, Oesterhelt F, Gaub H E.1999a. Force spectroscopy on single xanthan molecules. Applied Physics A, 68: 407-410.
[188]	Li H, Rief M, Oesterhelt F, Gaub H E, Zhang X, Shen J.1999b. Single - molecule force spectroscopy on polysaccharides by AFM - nano-mechanical fingerprint of α-(1, 4)-linked polysaccharides. Chemical Physics Letters, 305: 197-201.
[189]	Li J, Fern'{a}ndez J M, Berne B J.2010. Water's role in the force-induced unfolding of ubiquitin. Proceedings of the National Academy of Sciences of the United States of America, 107: 19284-19289.
[190]	Li Y, Qiao H, Yan W, Zhang J, Xing C, Wang H, Zhang B, Tang J.2013. Molecular recognition force spectroscopy study of the dynamic interaction between aptamer GBI-10 and extracellular matrix protein tenascin-C on human glioblastoma cell. Journal of Molecular Recognition, 26: 46-50.
[191]	Li Y, Wang J, Xing C, Wang Z, Wang H, Zhang B, Tang J.2011. Molecular recognition force spectroscopy study of the specific lectin and carbohydrate interaction in a living cell. ChemPhysChem, 12: 909-912.
[192]	Liang J, Fern'{a}ndez J M.2011. Kinetic measurements on single-molecule disulfide bond cleavage. Journal of the American Chemical Society, 133: 3528-3534.
[193]	Lin J, Xu H, Wu Y, Tang M, McEwen G D, Liu P, Hansen D R, Gilbertson T A, Zhou A.2013. Investigation of free fatty acid associated recombinant membrane receptor protein expression in HEK293 cells using raman spectroscopy, calcium imaging, and atomic force microscopy. Analytical Chemistry, 85: 1374-1381.
[194]	Liu K, Song Y, Feng W, Liu N, Zhang W, Zhang X.2011. Extracting a single polyethylene oxide chain from a single crystal by a combination of atomic force microscopy imaging and single-molecule force spectroscopy: toward the investigation of molecular interactions in their condensed states. Journal of the American Chemical Society, 133: 3226-3229.
[195]	Liu N, Bu T, Song Y, Zhang W, Li J, Zhang W, Shen J, Li H.2010a. The nature of the force-induced conformation transition of dsDNA studied by using single molecule force spectroscopy. Langmuir, 26: 9491-9496.
[196]	Liu N, Chen Y, Peng B, Lin Y, Wang Q, Su Z, Zhang W, Li H, Shen J.2013. Single-molecule force spectroscopy study on the mechanism of RNA disassembly in tobacco mosaic virus. Biophysical Journal, 105: 2790-2800.
[197]	Liu N, Peng B, Lin Y,Su Z, Niu Z, Wang Q, Zhang W, Li H, Shen J.2010b. Pulling genetic RNA out of tobacco mosaic virus using single-molecule force spectroscopy. Journal of the American Chemical Society, 132: 11036-11038.
[198]	Liu S, Maoz R, Sagiv J.2004. Planned nanostructures of colloidal gold via self-assembly on hierarchically assembled organic bilayer template patterns with in-situ generated terminal amino functionality. Nano Letters, 4: 845-851.
[199]	Lü S, Dudek D M, Cao Y, Balamurali M M, Gosline J, Li H.2010. Designed biomaterials to mimic the mechanical properties of muscles. Nature, 4656: 69-73.
[200]	Lyons R E, Lesieur E, Kim M, Wong D C, Huson M G, Nairn K M, Brownlee A G, Pearson R D, Elvin C M.2007. Design and facile production of recombinant resilin-like polypeptides: Gene construction and a rapid protein purification method. Protein Engineering Design and Selection, 20: 25-32.
[201]	Lyubchenko Y L, Gall A A, Shlyakhtenko L S.2013. Visualization of DNA and protein-DNA complexes with atomic force microscopy. Methods in Molecular Biology, 1117: 367-384.
[202]	Madl J, Rhode S, Stangl H, Stockinger H, Hinterdorfer P, Schütz G J, Kada G.2006. A combined optical and atomic force microscope for live cell investigations. Ultramicroscopy, 106: 645-651.
[203]	Marchetti M, Wuite G, Roos W H.2016. Atomic force microscopy observation and characterization of single virions and virus-like particles by nano-indentation. Current Opinion in Virology, 18: 82-88.
[204]	Mari S A, Köster S, Bippes C A, Yildiz O, Kühlbrandt W, Müller D J.2010. pH{-}induced conformational change of the beta-barrel forming protein OmpG reconstituted into native E. coli lipids. Journal of Molecular Biology, 396: 610-616.
[205]	Mari S A, Pessoa J, Altieri S, Hensen U, Thomas L, Morais-Cabral J H, Müller D J.2011. Gating of the MlotiK1 potassium channel involves large rearrangements of the cyclic nucleotide-binding domains. Proceedings of the National Academy of Sciences of the United States of America, 108: 20802-20807.
[206]	Marszalek P E, Greenleaf W J, Li H, Oberhauser A F, Fern'{a}ndez J M.2000. Atomic force microscopy captures quantized plastic deformation in gold nanowires. Proceedings of the National Academy of Sciences of the United States of America, 97: 6282-6286.
[207]	Marszalek P E, Lu H, Li H, Carrion-Vazquez M, Oberhauser A F, Schulten K, Fern'{a}ndez J M.1999. Mechanical unfolding intermediates in titin modules. Nature, 402: 100-103.
[208]	Marszalek P E, Oberhauser A F, Pang Y P, Fern'{a}ndez J M.1998. Polysaccharide elasticity governed by chair-boat transitions of the glucopyranose ring. Nature, 396: 661-664.
[209]	Marszalek P E, Oberhauser A F, Li H, Fern'{a}ndez J M.2003. The force-driven conformations of heparin studied with single molecule force microscopy. Biophysical Journal, 85: 2696-2704.
[210]	Marszalek P, Li H, Fern'{a}ndez J M.2001. Fingerprinting polysaccharides with singlemolecule atomic force microscopy. Nature Biotechnology, 19: 258-262.
[211]	Marszalek P, Li H, Oberhauser A F, Fern'{a}ndez J M.2002. Chair-boat transitions in single polysaccharide molecules observed with force-ramp AFM. Proceedings of the National Academy of Sciences of the United States of America, 99: 4278-4283.
[212]	Mashaghi A, Kramer G, Bechtluft P, Zachmann-Brand B, Driessen A J, Bukau B, Tans S J.2013. Reshaping of the conformational search of a protein by the chaperone trigger factor. Nature, 500: 98-101.
[213]	Mathur A B, Collinsworth A M, Reichert W M, Kraus W E, Truskey G A.2001. Endothelial, cardiac muscle and skeletal muscle exhibit different viscous and elastic properties as determined by atomic force microscopy. Journal of Biomechanics, 34: 1545-1553.
[214]	Matzke R, Jacobson K, Radmacher M.2001. Direct high-resolution measurement of furrow stiffening during division of adherent cells. Nature Cell Biology, 3: 607-610.
[215]	Medalsy I D, Müller D J.2013. Nanomechanical properties of proteins and membranes depend on loading rate and electrostatic interactions. ACS Nano, 7: 2642-2650.
[216]	Medalsy I, Hensen U, Müller D J.2011. Imaging and quantifying chemical and physical properties of native proteins at molecular resolution by force-volume AFM. Angewandte Chemie International Edition, 50: 12103-12108.
[217]	Merkel R, Nassoy P, Leung A, Ritchie K, Evans E.1999. Energy landscapes of receptor-ligand bonds explored with dynamic force spectroscopy. Nature, 397: 50-53.
[218]	Mickler M, Dima R I, Dietz H, Hyeon C, Thirumalai D, Rief M.2007. Revealing the bifurcation in the unfolding pathways of GFP by using single-molecule experiments and simulations. Proceedings of the National Academy of Sciences of the United States of America, 104: 20268-20273.
[219]	Milani P, Gholamirad M, Trass J, Arnedo A, Boudaoud A, Argoul F, Hamant O.2011. In vivo analysis of local wall stiffness at the shoot apical meristem in Arabidopsis using atomic force microscopy. The Plant Journal, 67: 1116-1123.
[220]	Monserrate A, Casado S, Flors C.2014. Correlative atomic force microscopy and localization-based super-resolution microscopy: Revealing labelling and image reconstruction artefacts. Chem Phys Chem, 15: 647-650.
[221]	Mora-Montes H M, Netea M G, Ferwerda G, Lenardon M D, Brown G D, Mistry A R, Kullberg B J, O'Callaghan C A, Sheth C C, Odds F C, Brown A J, Munro C A, Gow N A.2011. Recognition and blocking of innate immunity cells by Candida albicans chitin. Infection and Immunity, 79: 1961-1970.
[222]	Moreno-Flores S, Benitez R, Vivanco Md, Luis Toca-Herrera J.2010. Stress relaxation and creep on living cells with the atomic force microscope: A means to calculate elastic moduli and viscosities of cell components. Nanotechnology, 21: 445101.
[223]	Moy V T, Florin E L, Gaub H E.1994. Intermolecular forces and energies between ligands and receptors. Science, 266: 257-259.
[224]	Moy V T, Florin E, Rief M, Lehmann H, Ludwig M, Gaub H E, Dornmair K.1995. Probing the forces between complementary strands of DNA with the atomic force microscope. SPIE, 2384: 2-12.
[225]	Müller D J, Baumeister W, Engel A.1999. Controlled unzipping of a bacterial surface layer with atomic force microscopy. Proceedings of the National Academy of Sciences of the United States of America, 96: 13170-13174.
[226]	Müller D J, Dufrêne Y F.2011. Atomic force microscopy a nanoscopic window on the cell surface. Trends in Cell Biology, 21: 461-469.
[227]	Müller D J, Helenius J, Alsteens D, Dufrêne Y.2009. Force probing surfaces of living cells to molecular resolution. Nature Chemical Biology, 5: 383-390.
[228]	Müller D J, Engel A.1999. Voltage and pH{-}induced channel closure of porin OmpF visualized by atomic force microscopy. Journal of Molecular Biology, 285: 1347-1351.
[229]	Müller D J, Hand G M, Engel A, Sosinsky G.2002. Conformational changes in surface structures of isolated Connexin26 gap junctions. EMBO Journal, 21: 3598-3607.
[230]	Nash M, Gaub H E.2012. Single-molecule adhesion of a stimuli-responsive oligo(ethylene glycol) copolymer to gold. ACS Nano, 6: 10735-10742.
[231]	Neuert G, Albrecht C H, Gaub H E.2007. Predicting the rupture probabilities of molecular bonds in series. Biophysical Journal, 93: 1215-1223.
[232]	Ng S P, Billings K S, Ohashi T, Allen M D, Best R B, Randles L G, Erickson H P, Clarke J .2007. Designing an extracellular matrix protein with enhanced mechanical stability. Proceedings of the National Academy of Sciences of the United States of America, 104: 9633-9637.
[233]	Nunes J M, Hensen U, Ge L, Lipinsky M, Helenius J, Grubmüller H, Müller D J.2010. A "force buffer" protecting immunoglobulin titin. Angewandte Chemie International Edition, 49: 3528-3531.
[234]	Nunes J M, Mayer-Hartl M, Hartl F U, Müller D J.2015. Action of the Hsp70 chaperone system observed with single proteins. Nature Communications, 6: 6307.
[235]	Oberhauser A F, Hansma P K, Carrion-Vazquez M, Fern'{a}ndez J M.2001. Stepwise unfolding of titin under force-clamp atomic force microscopy. Proceedings of the National Academy of Sciences of the United States of America, 98: 468-472.
[236]	Oberhauser A F, Marszalek P E, Carrion-Vazquez M, Fern'{a}ndez J M.1999. Single protein misfolding events captured by atomic force microscopy. Nature Structural & Molecular Biology, 6: 1025-1028.
[237]	Oberhauser A F, Marszalek P E, Erickson H P, Fern'{a}ndez J M.1998. The molecular elasticicty of the extraclleuar matrix protein tenascin. Nature, 393: 181-185.
[238]	O'Donoghue M B, Shi X, Fang X, Tan W.2012. Single-molecule atomic force microscopy on live cells compares aptamer and antibody rupture forces. Analytical and Bioanalytical Chemistry, 402: 3205-3209.
[239]	Oesterhelt F, Oesterhelt D, Pfeiffer M, Engel A, Gaub H E, Müller D J.2000. Unfolding pathways of individual bacteriorhodopsins. Science, 288: 143-146.
[240]	Osada T, Itoh A, Ikai A.2003. Mapping of the receptor-associated protein (rap) binding proteins on living fibroblast cells using an atomic force microscope. Ultramicroscopy, 97: 353-357.
[241]	Paik D H, Perkins T T.2011. Overstretching DNA at 65 pN does not require peeling from free ends or nicks. Journal of the American Chemical Society, 133: 3219-3221.
[242]	Park J W, Park A Y, Lee S, Yu N K, Lee S H, Kaang B K.2010. Detection of TRKB receptors distributed in cultured hippocampal neurons through bioconjugation between highly luminescent (quantum dot-neutravidin) and (biotinylated anti-TRKB antibody) on neurons by combined atomic force microscope and confocal laser scanning microscope. Bioconjugate Chemistry, 21: 597-603.
[243]	Park P S, Sapra K T, Koli'{n}ski M, Filipek S, Palczewski K, Müller D J.2007. Stabilizing effect of Zn(^{2 + }) in native bovine rhodopsin. Journal of Biological Chemistry, 282: 11377-11385.
[244]	Paul P C, Knoll A W, Holzner F, Despont M, Duerig U.2011. Rapid turnaround scanning probe nanolithography. Nanotechnology, 22: 275306.
[245]	Peng Q, Li H.2008. Atomic force microscopy reveals parallel mechanical unfolding pathways of T4 lysozyme: evidence for a kinetic partitioning mechanism. Proceedings of the National Academy of Sciences of the United States of America, 105: 1885-1890.
[246]	Peng Q, Li H.2009a. Direct Observation of tug-of-war during the folding of a mutually exclusive protein. Journal of the American Chemical Society, 131: 13347-13354.
[247]	Peng Q, Li H.2009b. Domain insertion effectively regulates the mechanical unfolding hierarchy of elastomeric proteins toward engineering multifunctional elastomeric proteins. Journal of the American Chemical Society, 131: 14050-14056.
[248]	Perez-Jimenez R, Alonso-Caballero A, Berkovich R, Franco D, Chen M W, Richard P, Badilla CL, Fern'{a}ndez J M.2014. Probing the effect of force on HIV{-}1 receptor CD4. ACS Nano, 8: 10313-10320.
[249]	Perez-Jimenez R, Garcia-Manyes S, Ainavarapu S R, Fern'{a}ndez J M.2006. Mechanical unfolding pathways of the enhanced yellow fluorescent protein revealed by single molecule force spectroscopy. Journal of Biological Chemistry, 281: 40010-40014.
[250]	Perez-Jimenez R, Ingl'{e}s-Prieto A, Zhao Z M, Sanchez-Romero I, Alegre-Cebollada J, Kosuri P, Garcia-Manyes S, Kappock T J, Tanokura M, Holmgren A, Sanchez-Ruiz J M, Gaucher E A, Fern'{a}ndez J M.2011. Single-molecule paleoenzymology probes the chemistry of resurrected enzymes. Nature Structural & Molecular Biology, 18: 592-596.
[251]	Perez-Jimenezand R, Li J, Kosuri P, Sanchez-Remero I, Wiita A P, Rodriguez-Larrea D, Chueca A, Holmgren A, Miranda-Vizuete A, Becker K, Cho S, Beckwith J, Gelhaye E, Jacquot J P, Gaucher E A, Sanchez-Ruiz J M, Berne B J, Fern'{a}ndez J M.2009. Diversity of chemical mechanisms in thioredoxin catalysis revealed by single-molecule force spectroscopy. Nature Structural & Molecular Biology, 16: 890-896.
[252]	Pernigo S, Fukuzawa A, Bertz M, Holt M, Rief M, Steiner R A, Gautel M P.2010. Structural insight into M-band assembly and mechanics from the titin-obscurin-like-1 complex. Proceedings of the National Academy of Sciences of the United States of America, 107: 2908-2913.
[253]	Pfreundschuh M, Alsteens D, Hilbert M, Steinmetz M O, Müller D J.2014. Localizing chemical groups while imaging single native proteins by high-resolution atomic force microscopy. Nano Letters, 14: 2957-2964.
[254]	Pfreundschuh M, Alsteens D, Wieneke R, Zhang C, Coughlin S R, Tamp'{e} R, Kobilka B K, Müller D J.2015. Identifying and quantifying two ligand-binding sites while imaging native human membrane receptors by AFM. Nature Communications, 6: 8857.
[255]	Pfreundschuh M, Hensen U, Müller D J.2013. Quantitative imaging of the electrostatic field and potential generated by a transmembrane protein pore at subnanometer resolution. Nano Letters, 13: 5585-5593.
[256]	Pires D, Hedrick J L, De Silva A, Frommer J, Gotsmann B, Wolf H, Despont M, Duerig U, Knoll A W2010. Nanoscale three-dimensional patterning of molecular resists by scanning probes. Science, 328: 732-735.
[257]	Plodinec M, Loparic M, Aebi U.2010. Imaging articular cartilage tissue using atomic force microscopy (AFM). Cold Spring Harb Protocols, 2010: pdb.prot5499.
[258]	Popa I, Berkovich R, Alegre-Cebollada J, Badilla C L, Rivas-Pardo J A, Taniguchi Y, Kawakami M, Fern'{a}ndez J M.2013a. Nanomechanics of HaloTag tethers. Journal of the American Chemical Society, 135: 12762-12771.
[259]	Popa I, Kosuri P, Alegre-Cebollada J, Garcia-Manyes S, Fern'{a}ndez J M.2013b. Force dependency of biochemical reactions measured by single-molecule force-clamp spectroscopy. Nature Protocols, 8: 1261-1276.
[260]	Puchner E M, Alexandrovich A, Kho A L, Hensen U, Schafer L V, Brandmeier B, Grater F, Grubmüller H, Gaub H E, Gautel M .2008. Mechanoenzymatics of titin kinase. Proceedings of the National Academy of Sciences of the United States of America, 105: 13385-13390.
[261]	Puchner E M, Gaub H E.2012. Single-molecule mechanoenzymatics. Annual Review of Biophysics, 41: 497-518.
[262]	Puchner E M, Gaub H E.2009. Force and function: Probing proteins with AFM-based force spectroscopy. Current Opinion in Structural Biology, 19: 605-614.
[263]	Puntheeranurak T, Wimmer B, Castaneda F, Gruber H J, Hinterdorfer P, Kinne R K.2007. Substrate specificity of sugar transport by rabbit SGLT1. Biochemistry, 46: 2797-2804.
[264]	Pyne A, Thompson R, Leung C, Roy D, Hoogenboom B W.2014. Single-molecule reconstruction of oligonucleotide secondary structure by atomic force microscopy. Small, 10: 3257-3261.
[265]	Qiao H, Krajcikova D, Liu C, Li Y, Wang H, Barak I, Tang J.2012. The interactions of spore coat morphogenetic proteins studied by single molecule recognition force spectroscopy. Chemistry - An Asian Journal, 7: 725-731.
[266]	Ramanathan S P, Helenius J, Stewart M P, Cattin C J, Hyman A A, Müller D J.2015. Cdk1-dependent mitotic enrichment of cortical myosin II promotes cell rounding against confinement. Nature Cell Biology, 17: 148-159.
[267]	Rangl M, Miyagi A, Kowal J, Stahlberg H, Nimigean C M, Scheuring S.2016. Real-time visualization of conformational changes within single MloK1 cyclic nucleotide-modulated channels. Nature Communications, 7: 12789.
[268]	Rico F, Gonzalez L, Casuso I, Puig-Vidal M, Scheuring S.2013. High-speed force spectroscopy unfolds titin at the velocity of molecular dynamics simulations. Science, 342: 741-743
[269]	Rief M, Clausen-Schaumann H, Gaub H E.1999. Sequence-dependent mechanics of single DNA molecules. Nature Structural & Molecular Biology, 6: 346-349.
[270]	Rief M, Oesterhelt F, Heymann B, Gaub H E.1997a. Single molecule force spectroscopy on polysaccharides by atomic force microscopy. Science, 275: 1295-1297.
[271]	Rief M, Gautel M, Oesterhelt F, Fern'{a}ndez J M, Gaub H E.1997b. Reversible unfolding of individual titin immunoglobulin domains by AFM. Science, 276: 1109-1112.
[272]	Sader J E.2002. Calibration of Atomic Force Microscope Cantilevers. Encyclopedia of Surface and Colloid Science.
[273]	Sader J E, Chon J, Mulvaney P.1999. Calibration of rectangular atomic force microscope cantilevers. The Review of Scientific Instruments, 70: 3967-3969.
[274]	Sakiyama Y, Mazur A, Kapinos L E, Lim R Y.2016. Spatiotemporal dynamics of the nuclear pore complex transport barrier resolved by high-speed atomic force microscopy. Nature Nanotechnology, 11: 719-723.
[275]	Santosa N, Castanhob M.2004. An overview of the biophysical applications of atomic forcemicroscopy. Biophysical Chemistry, 107: 133-149.
[276]	Sapra T K, Damaghi M, Köster S, Yildiz O, Kühlbrandt W, Müller D J.2009. One b hairpin after the other: exploring mechanical unfolding pathways of the transmembrane b-barrel protein OmpG. Angewandte Chemie International Edition, 48: 8306-8308.
[277]	Sapra T K, Park P S, Filipek S, Engel A, Müller D J, Palczewski K.2006. Detecting molecular interactions t hat stabilize native bovine rhodopsin. Journal of Molecular Biology, 358: 255-269.
[278]	Sasuga S, Abe R, Nikaido O, Kiyosaki S, Sekiguchi H, Ikai A, Osada T.2012. Interaction between pheromone and its receptor of the fission yeast Schizosaccharomyces pombe examined by a force spectroscopy study. Journal of Biomedicine and Biotechnology, 2012: 804793.
[279]	Saulson P R.1990. Thermal noise in mechanical experiments. Physical Review D, 42: 2437-2445.
[280]	Schlierf M, Li H, Fern'{a}ndez J M.2004. The unfolding kinetics of ubiquitin captured with single-molecule force-clamp techniques. Proceedings of the National Academy of Sciences of the United States of America, 101: 7299-7304.
[281]	Schlierf M, Rief M.2006. Single-molecule unfolding force distributions reveal a funnel-shaped energy landscape. Biophysical Journal, 90: 33-35.
[282]	Schoeler C, Bernardi R C, Malinowska K H, Durner E, Ott W, Bayer E A, Schulten K, Nash M A, Gaub H E.2015. Mapping mechanical force propagation through biomolecular complexes. Nano Letters, 15: 7370-7376.
[283]	Seelert H, Poetsch A, Dencher N A, Engel A, Stahlberg H, Müller D J.2000. Proton-powered turbine of a plant motor. Nature, 405: 418-419.
[284]	Serdiuk T, Balasubramaniam D, Sugihara J, Mari S A, Kaback H R, Müller D J.2016. YidC assists the stepwise and stochastic folding of membrane proteins. Nature Chemical Biology, 12: 911-917.
[285]	Serdiuk T, Madej M G, Sugihara J, Kawamura S, Mari S A, Kaback H R, Müller D J.2014. Substrate-induced changes in the structural properties of LacY. Proceedings of the National Academy of Sciences of the United States of America, 111: 1571-1580.
[286]	Shan Y, Huang J, Tan J, Gao G, Liu S, Wang H, Chen Y.2012. The study of single anticancer peptides interacting with HeLa cell membranes by single molecule force spectroscopy. Nanoscale, 4: 1283-1286.
[287]	Sharma S, Grintsevich E E, Woo J, Gurel P S, Higgs H N, Reisler E, Gimzewski J K.2014. Nanostructured self-assembly of inverted formin 2 (INF2) and F-actin-INF2 complexes revealed by atomic force microscopy. Langmuir, 30: 7533-7539.
[288]	Sheng X, Ting Y P, Pehkonen S O.2007. Force measurements of bacterial adhesion on metals using a cell probe atomic force microscope. Journal of Colloid and Interface Science, 310: 661-669.
[289]	Shi L, Howan K, Shen Q T, Wang Y J, Rothman J E, Pincet F.2013. Preparation and characterization of SNARE-containing nanodiscs and direct study of cargo release through fusion pores. Nature Protocols, 8: 935-948.
[290]	Shi X, Xu L, Yu J, Fang X.2009. Study of inhibition effect of Herceptin on interaction between Heregulin and ErbB receptors HER3/HER2 by single-molecule force spectroscopy. Experimental Cell Research , 315: 2847-2855.
[291]	Shibata M, Uchihashi T, Yamashita H, Kandori H, Ando T.2011. Structural changes in bacteriorhodopsin in response to alternate illumination observed by high-speed atomic force microscopy. Angewandte Chemie International Edition, 123: 4502-4505.
[292]	Shinto H, Aso Y, Fukasawa T, Higashitani K.2012. Adhesion of melanoma cells to the surfaces of microspheres studied by atomic force microscopy. Advanced Powder Technology, 23: 114-121.
[293]	Skulason H.2002. Direct detection of intermolecular forces by atomic force microscopy. [PhD Thesis]. USA: University of Minnesota.
[294]	Smith B, Schäffer T, Viani M, Thompson J, Frederick N, Kindt J,Belcher A, Stucky G, Morse D, Hansma P.1999. Molecular mechanistic origin of the toughness of natural adhesives fibres and composites. Nature, 399: 761-763.
[295]	Smith S B, Finzi L, Bustamante C.1992. Direct mechanical measurements of the elasticity of single DNA-molecules by using magnetic beads. Science, 258: 1122-1126.
[296]	Sneddon I N.1965. The relation between load and penetration in the axisymmetric Boussinesq problem for a punch of arbitrary profile. International Journal of Engineering Science, 3: 47-57.
[297]	Song Y, Feng W, Liu K, Yang P, Zhang W, Zhang X.2013. Exploring the folding pattern of a polymer chain in a single crystal by combining single-molecule force spectroscopy and steered molecular dynamics simulations. Langmuir, 29: 3853-3857.
[298]	Sonnenberg L, Luo Y, Schlaad H, Seitz M, Cölfen H, Gaub H E.2007b. Quantitative single molecule measurements on the interaction forces of poly(L-glutamic acid) with calcite crystals. Journal of the American Chemical Society, 129: 15364-15371.
[299]	Sonnenberg L, Parvole J, Kühner F, Billon L, Gaub H E.2007a. Choose Sides: Differential Polymer Adhesion. Langmuir, 23: 6660-6666.
[300]	Stahl S W, Puchner E M, Gaub H E.2009. Photothermal cantilever actuation for fast single-molecule force spectroscopy. The Review of Scientific Instruments, 80: 73702.
[301]	Stamov D R, Stock E, Franz C M, Jahnke T, Haschke H.2015. Imaging collagen type I fibrillogenesis with high spatiotemporal resolution. Ultramicroscopy, 149: 86-94.
[302]	Stewart M P, Helenius J, Toyoda Y, Ramanathan S P, Müller D J, Hyman A A.2011. Hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding. Nature, 469: 226-230.
[303]	Stewart M P, Hodel A W, Spielhofer A, Cattin C J, Müller D J, Helenius J.2013. Wedged AFM-cantilevers for parallel plate cell mechanics. Methods, 60: 186-194.
[304]	Stewart M P, Toyoda Y, Hyman A A, Müller D J.2012. Tracking mechanics and volume of globular cells with atomic force microscopy using a constant-height clamp. Nature Protocols, 7: 143-154.
[305]	Strackharn M, Pippig D A, Meyer P, Stahl S W, Gaub H E.2012. Nanoscale arrangement of proteins by single-molecule cut-and-paste. Journal of the American Chemical Society, 134: 15193-15196.
[306]	Strilić B, Eglinger J, Krieg M, Zeeb M, Axnick J, Bab'{a}l P, Müller D J, Lammert E.2010. Electrostatic cell-surface repulsion initiates lumen formation in developing blood vessels. Current Biology, 20: 2003-2009.
[307]	Strunz T, Oroszlan K, Schumakovitch I, Güntherodt H, Hegner M.2000. Model energy landscapes and the force-induced dissociation of ligand-rceptor bonds. Biophysical Journal, 79: 1206-1212.
[308]	Sullan R M A, Churnside A B, Nguyen D M, Bull M S, Perkins T T.2013. Atomic force microscopy with sub-picoNewton force stability for biological applications. Methods, 60: 131-141.
[309]	Te Riet J, Helenius J, Strohmeyer N, Cambi A, Figdor C G, Müller D J.2014. Dynamic coupling of ALCAM to the actin cortex strengthens cell adhesion to CD6. Journal of Cell Science, 127: 1595-1606.
[310]	Thie M, Röspel R, Dettmann W, Benoit M, Ludwig M, Gaub H E, Denker H W.1998. Interactions between trophoblast and uterine epithelium: monitoring of adhesive forces. Human Reproduction, 11: 3211-3219.
[311]	Thoma J, Bosshart P, Pfreundschuh M, Müller D J. 2012. Out but not in: The large transmembrane b-barrel protein FhuA unfolds but cannot refold via b-hairpins. Structure, 20: 2185-2190.
[312]	Thoma J, Burmann B, Hiller S, Müller D J.2015. Impact of holdase chaperones Skp and SurA on the folding of β-barrel outer-membrane proteins. Nature Structural & Molecular Biology, 22: 795-802.
[313]	Uchihashi T, Iino R, Ando T, Noji H.2011. High-speed atomic force microscopy reveals rotary catalysis of rotorless F1-ATPase. Science, 333: 755-758.
[314]	Ulrich F, Krieg M, Schötz EM, Link V, Castanon I, Schnabel V, Taubenberger A, Müller D, Puech P H, Heisenberg C P.2005. Wnt11 functions in gastrulation by controlling cell cohesion through Rab5c and E{-}cadherin. Developmental Cell, 9: 555-564.
[315]	Viani M B, Pietrasanta L I, Thompson J B, Chand A, Gebeshuber I C, Kindt J H, Richter M, Hansma H G, Hansma P K.2000. Probing protein-protein interactions in real time. Nature Structural & Molecular Biology, 7: 644-647.
[316]	Volle C B, Ferguson M A, Aidala K E, Spain E M, N'{u} {n}ez M E.2008. Quantitative changes in the elasticity and adhesive properties of Escherichia coli ZK1056 prey cells during predation by Bdellovibrio bacteriovorus 109J. Langmuir, 24: 8102-8110.
[317]	Walther K A, Gräter F, Dougan L, Badilla C L, Berne B J, Fern'{a}ndez J M.2007. Signatures of hydrophobic collapse in extended proteins captured with force spectroscopy. Proceedings of the National Academy of Sciences of the United States of America, 104: 7916-7921.
[318]	Wang H, Obenauer-Kutner L, Lin M, Huang Y, Grace M, Lindsay S.2008. Imaging glycosylation. Journal of the American Chemical Society, 130: 8154-8155.
[319]	Wang M S, Palmer L B, Schwartz J D, Razatos A.2004. Evaluating protein attraction and adhesion to biomaterials with the atomic force microscope. Langmuir, 20: 7753-7759.
[320]	Wang M, Cao Y, Li H.2006. The unfolding and folding dynamics of TNfnALL probed by single molecule force-ramp spectroscopy. Polymer, 47: 2548-2554.
[321]	Watanabe-Nakayama T, Itami M, Kodera N, Ando T, Konno H.2016. High-speed atomic force microscopy reveals strongly polarized movement of clostridial collagenase along collagen fibrils. Scientific Reports, 6: 28975.
[322]	Watson J D, Crick F H.1953. Molecular structure of nucleic acids; a structure for deoxyribose nucleic acid. Nature, 171: 737-738.
[323]	Weafer P P, McGarry J P, van Es M H, Kilpatrick J I, Ronan W, Nolan D R, Jarvis S P.2012. Stability enhancement of an atomic force microscope for long-term force measurement including cantilever modification for whole cell deformation. The Review of Scientific Instruments, 83: 93709.
[324]	Wegmann S, Medalsy I D, Mandelkow E, Müller D J.2013. The fuzzy coat of pathological human Tau fibrils is a two-layered polyelectrolyte brush. Proceedings of the National Academy of Sciences of the United States of America, 110: 313-321.
[325]	Weiner G J.2010. Rituximab: Mechanism of action. Seminars in Hematology, 47: 115-123.
[326]	Whitehead K A, Rogers D, Colligon J, Wright C, Verran J.2006. Use of the atomic force microscope to determine the effect of substratum surface topography on the ease of bacterial removal. Colloids and Surfaces B: Biointerfaces, 51: 44-53.
[327]	Wiita A P, Ainavarapu S R, Huang H H, Fern'{a}ndez J M.2006. Force-dependent chemical kinetics of disulfide bond reduction observed with single-molecule techniques. Proceedings of the National Academy of Sciences of the United States of America, 103: 7222-7227.
[328]	Wiita A P, Perez-Jimenez R, Walther K A, Gräter F, Berne B J, Holmgren A, Sanchez-Ruiz J M, Fern'{a}ndez J M.2007. Probing the chemistry of thioredoxin catalysis with force. Nature, 450: 124-127.
[329]	Wildling L, Rankl C, Haselgrübler T, Gruber H J, Holy M, Newman A H, Zou MF, Zhu R, Freissmuth M, Sitte H H, Hinterdorfer P.2012. Probing binding pocket of serotonin transporter by single molecular force spectroscopy on living cells. Journal of Biological Chemistry, 287: 105-113.
[330]	Wildling L, Unterauer B, Zhu R, Rupprecht A, Haselgrübler T, Rankl C, Ebner A, Vater D, Pollheimer P, Pohl E E, Hinterdorfer P, Gruber H J.2011. Linking of sensor molecules with amino groups to amino-functionalized AFM tips. Bioconjugate Chemistry, 26: 1239-1248.
[331]	Wong J Y, McDonald J, Taylor-Pinney M, Spivak D I, Kaplan D L, Buehler M J .2012. Materials by design: Merging proteins and music. Nano Today, 7: 488-495.
[332]	Woodside M T, Block S M.2014. Reconstructing folding energy landscapes by single-molecule force spectroscopy. Annual Review of Biophysics, 43: 19-39.
[333]	Xue Y, Li X, Li H, Zhang W.2014. Quantifying thiol-gold interactions towards the efficient strength control. Nature Communications, 5: 4348.
[334]	Yamada K M, Cukierman E.2007. Modeling tissue morphogenesis and cancer in 3D. Cell, 130: 601-610.
[335]	Yamashita H, Taoka A, Uchihashi T, Asano T, Ando T, Fukumori Y.2012. Single-molecule imaging on living bacterial cell surface by high-speed AFM. Journal of Molecular Biology, 422: 300-309.
[336]	Yu J, Jiang Y, Ma X, Lin Y, Fang X.2007a. Energy landscape of aptamer/protein complexes studied by single-molecule force spectroscopy. Chemistry - An Asian Journal, 2: 284-289.
[337]	Yu J, Wang Q, Shi X, Ma X, Yang H, Chen Y G, Fang X.2007b. Single-molecule force spectroscopy study of interaction between transforming growth factor β1 and its receptor in living cells. The Journal of Physical Chemistry B, 111: 13619-13625.
[338]	Zhang J, Wu G, Song C, Li Y, Qiao H, Zhu P, Hinterdorfer P, Zhang B, Tang J.2012. Single molecular recognition force spectroscopy study of a luteinizing hormone-releasing hormone analogue as a carcinoma target drug. The Journal of Physical Chemistry B, 116: 13331-13337.
[339]	Zhang S, Andreasen M, Nielsen JT, Liu L, Nielsen EH, Song J, Ji G, Sun F, Skrydstrup T, Besenbacher F, Nielsen N C, Otzen D E, Dong M.2013. Coexistence of ribbon and helical fibrils originating from hIAPP(20-29) revealed by quantitative nanomechanical atomic force microscopy. Proceedings of the National Academy of Sciences of the United States of America, 110: 2798-2803.
[340]	Zhang S, Aslan H, Besenbacher F, Dong M.2014. Quantitative biomolecular imaging by dynamic nanomechanical mapping. Chemical Society Reviews, 43: 7412-7429.
[341]	Zhang T, Chao Y, Shih K, Li X Y, Fang H.2011. Quantification of the lateral detachment force for bacterial cells using atomic force microscope and centrifugation. Ultramicroscopy, 111: 131-139.
[342]	Zhang W, Barbagallo R, Madden C, Roberts C J, Woolford A, Allen S.2005. Progressing single biomolecule force spectroscopy measurements for the screening of DNA binding agents. Nanotechnology, 16: 2325-2333.
[343]	Zhang W, Lü X, Zhang W, Shen J.2011. EMSA and single-molecule force spectroscopy study of interactions between bacillus subtilis single-stranded DNA-binding protein and single-stranded DNA. Langmuir, 27: 15008-15015.
[344]	Zhang W, Xu Q, Zou S, Li H, Xu W, Zhang X, Shao S, Kudera M, Gaub H E.2000. Single-molecule force spectroscopy on bombyx mori silk fibroin by atomic force microscopy. Langmuir, 16: 4305-4308.
[345]	Zhang X, Bogorin D F, Moy V T.2004. Molecular basis of the dynamic strength of the sialyl Lewis X-selectin interaction. ChemPhysChem, 5: 175-182.
[346]	Zhang Y H, Liu C J, Shi W Q, Wang Z Q, Dai L M, Zhang X.2007. Direct measurements of the interaction between pyrene and graphite in aqueous media by single molecule force spectroscopy: Understanding the pi-pi interactions. Langmuir, 23: 7911-7915.
[347]	Zhao W, Cai M, Xu H, Jiang J, Wang H.2013. A single-molecule force spectroscopy study of the interactions between lectins and carbohydrates on cancer and normal cells. Nanoscale, 5: 3226-3229.
[348]	Zheng P, Arantes G, Field M, Li H.2015. Force-induced chemical reactions on the metal centre in a single metalloprotein molecule. Nature Communications, 6: 7569.
[349]	Zheng P, Chou C C, Guo Y, Wang Y, Li H.2013b. Single molecule force spectroscopy reveals the molecular mechanical anisotropy of the FeS₄ metal center in rubredoxin. Journal of the American Chemical Society, 135: 17783-17792.
[350]	Zheng P, Takayama S J, Mauk A G, Li H.2013a. Single molecule force spectroscopy reveals that iron is released from the active site of rubredoxin by a stochastic mechanism. Journal of the American Chemical Society, 135: 7992-8000.
[351]	Zheng P, Wang Y, Li H.2014. Reversible Unfolding-refolding of rubredoxin a single-molecule force spectroscopy study. Angewandte Chemie International Edition, 53: 14060-14063.
[352]	Zhu R, Howorka S, Pröll J, Kienberger F, Preiner J, Hesse J, Ebner A, Pastushenko V P, Gruber H J, Hinterdorfer P.2010. Nanomechanical recognition measurements of individual DNA molecules reveal epigenetic methylation patterns. Nature Nanotechnology, 5: 788-791.
[353]	Zhu R, Rupprecht A, Ebner A, Haselgrübler T, Gruber H J, Hinterdorfer P, Pohl E E.2013. Mapping the nucleotide binding site of uncoupling protein 1 using atomic force microscopy. Journal of the American Chemical Society, 135: 3640-3646.
[354]	Zocher M, Roos C, Wegmann S, Bosshart P D, Dötsch V, Bernhard F, Müller D J.2012b. Single-molecule force spectroscopy from nanodiscs: An assay to quantify folding stability and interactions of native membrane proteins. ACS Nano, 6: 961-971.
[355]	Zocher M, Zhang C, Rasmussen S G, Kobilka B K, Müller D J.2012a. Cholesterol increases kinetic, energetic, and mechanical stability of the human β2-adrenergic receptor. Proceedings of the National Academy of Sciences of the United States of America, 109: 3463-3472